Technologies

The Unit of Cytometry of GENYO has available the following equipment to support scientific community and develop the type of research studies described above:

BD FACTSCanto II Flow Cytometer

Trademark: BD Biosciences
Technical Specifications: Technical Specifications: It is equipped with a solid state Coherent® Sapphire™ air-cooled blue laser (488-nm, 20-mW) able to detect 4 colors; JDS Uniphase™ HeNe (633 nm y 17 mW) red laser with 2 colors. It is equipped with a total of 8 detectors (6 fluorescence detectors and 2 morphologic parameters). It runs with the FACSDiva software. The system is equipped with a module of quality control that determines the optimal voltages and checks the system for best performance.

BD FACSVerse™ Flow Cytometer

Trademark: BD Biosciences
Technical Specifications: It is used in investigation. It is provided with three laser lines: blue laser (488-nm) for 4 colors, a red laser (633nm) for 2 colors, and a violet laser (407nm) for 2 colors. It is able to detect up to 10 dispersion parameters (8 fluorescence detectors and 2 morphologic parameters). It presents a laser system designed to minimize light losses and maximize resolution for multiparametric applications. It has a micro motor for automated laser alignment. This cytometer presents standard acquisition velocity rates (high, medium, and low), and a high-sensitivity mode making easier the detection of dimly staining particles. The fluidic system allows handling acquisition of samples in any tube format. It has a sample loader able to analyze from 30-40 tubes in a single run to 96 and 384 well-plates. It has volumetric counting. It runs with the FACSuite software. The system is equipped with a module of quality control that determines the optimal voltages and checks the system for best performance to maximize reliability of results.

BD FACSAria Cell Sorter

Trademark: BD Bioscencies
Technical Specifications: : It is provided with three laser lines: The Coherent ® Sapphire ™ solid state blue laser (488 nm, 13 mW) can detect 5 colors; The Coherent Sapphire diode-pumped solid-state blue laser (488 nm, 13 mW) is able to detect 5 colors; JDS Uniphase™ air-cooled HeNe (633 nm y 17 mW) red laser for 2 colors (633 nm, 11mW), and solid-state violet laser (407 nm and 10 mW) for 2 colors. It is able to detect up to 11 dispersion parameters simultaneously (9 fluorescence detectors, Forward Scatter (FSC) and Side scatter (SSC)), as well as to purify different types of cell populations based on specific features. Acquisition velocity rate can reach up to 70,000 events per second. It accepts different supports for samples acquisition and sorting. It is equipped with ACDU (Automated Cell Deposition Unit) Unit for sorting into multi-well plates or slides. The FACSAria is equipped with a refrigeration system that allows samples acquisition and sorting with controlled temperature. It runs with the FACSuite software for sample acquisition and analysis.