Equipment

Equipment

The Unit is equipped with Epi-fluorescence microscopy, confocal laser microscopy, and laser capture microdissection systems. The main characteristics of all of them are the following:

Epi-fluorescence Microscopes:

  • Nikon Eclipse 50i Microscope:

    Upright microscope with 10x, 20x, 40x (dry objectives) and 100x (oil immersion objectives), transmitted light techniques (bright field, phase contrast, and DIC) and DAPI, FITC y TxRed filters.

  • Nikon Eclipse 80i Microscope:

    Upright microscope with 10x, 40x (dry objectives) and 100x (oil immersion objectives), transmitted light techniques (bright field, and DIC) and DAPI, FITC, TxRed, FITC/TxRed, Sp.GOLD, and Sp.AQUA filters.

  • Nikon Eclipse 90i ProScanIII Microscope:

    Upright microscope with spectral camera 420-720 nm, and 2x, 10x, 20x y 40x dry objectives, transmitted light techniques (bright field, and DIC) and DAPI, FITC, E-94 (orange) and E-93 (red) fully motorized.

  • Nikon Eclipse TE2000-U Microscope,

    Inverted microscope with 4x, 10x,20x,40x, and 60x objectives (dry-type), transmitted light techniques (bright field, and phase contrast) and DAPI, FITC and TxRed filters.

  • Zeiss Axio Imager A.1 Microscope:

    Upright microscope with 5x, 10x, 20x (dry objectives), 63x, and 100x (oil immersion objectives), transmitted light techniques (bright field, and phase contrast) and BFP, DAPI, A488, and Rhod filters.

Confocal Laser Microscope

Confocal Laser Microscope  Inverted microscope with 5 excitation laser lines: diode laser (405 nm), Argon laser (458 nm, 488 nm, 514 nm), HeNe laser (543 nm, 594 nm, and 633 nm); with 10x, 20x dry objectives, 40x, 63x oil immersion objectives,63x water and glycerol immersion, transmitted light techniques (bright field, and DIC), Zeiss filters for DAPI, GFP, DsRed, and FITC-TRITC, with 3 internal or photomultiplier detection channels (PMTs); the system is fully motorized, with CO2 and temperature controlled incubation system for “in vivo” assays.

    

Zeiss PALM MicroBeam IV Laser Microdissection instrument

Inverted microdissection system with one excitation laser line of ultraviolet range (355nm), objectives 10x,20x,40x and 63x (dry-type), transmitted light techniques (bright field, phase contrast, and DIC); DAPI, GFP and Rhod filter set; the system is fully motorized, with high precision cutting and catapulting, and “cap check” system.

    

Processing Software and Imaging Analysis: