List of services

List of Services::


1) Scientific Advice

2) High-throughput Microarray s Platforms. iScan (Illumina) and High-Resolution C Scanner (Agilent)

  • SNPs Genotyping and Identification of CNV
  • Whole-genome expression analysis
  • Transcription and methylation studies
  • Cytogenetic analysis
  • Linkage studies
  • Whole-genome genotyping.
  • Regulation analysis and epigenetics
  • See full list
3) Next Generation Sequencing (SOLID).

  • Genomics:
    • Targeted resequencing (exome, specific genes, long-range PCR ...)
    • Whole genome resequencing (human genome, mitochondrial DNA, microbial ...)
    • Novo sequencing
  • Expression:
    • Gene expression analysis
    • Full transcriptome analysis (RNA-Seq)
    • Small RNAs analysis (miRNAs)
  • Epigenetics:
    • Methylation analysis
    • Analysis of DNA-protein interactions (ChIP-Seq)
    • Study RNA-protein interactions (CLIP-Seq)
  • See full list
 
4) Capillary sequencing and DNA fragments analysis

  • PCR sequencing products, plasmids and cosmids.
  • Characterisation by PCR product size (study of microsatellites, STRs, and minisatellites).
  • Editing and reading of sequences and fragments.
  • See recommendations
  • See full list
5) Quantitative real-time PCR.

  • Optimal design of primers and probes for quantitative PCR assays.
    • Optimal design of primers and probes for quantitative PCR assays.
    • Development of quantitative real-time PCR
    • Data Analysis
  • Determination of the number of copies of repetitive regions.
  • Absolute quantification.
  • See full list

Rt-PCR:

  • Selection of candidate genes: the researcher can chose which genes want to study and request to the Service of the Unit the genes available in the database of the Service according to the biological function, cell localization, chromosomal position, etc
  • Primers design: according to whether the user want to amplify cDNA, genomic DNA,…
  • Gene expression analysis: determining transcript expression levels by real time RT-PCR.
  • Variations in splicing: selection of annotated exons, primers design, RT-PCR and electrophoresis.
  • SNPs genotyping for allelic discrimination studies, referring to concrete SNPs and specific genes or regions where it will be necessary the search of SNPs and probes design on the part of the Service of the Unit among more than 4.5 million characterized SNPs.
  • Estimation of the number of copies of a specific gene.
  • MicroRNAs analysis: by the use of specific primers.

An overview of these products can be found below:

Placas TaqMan® (96-384 Arrays). Más de 270 placas prediseñadas, algunas de ellas:

  • Human p53 Signalin
  • Human Telomere Extension by Telomerase
  • Human DNA Methylation And Transcriptional Repression
  • Human Growth Factors
  • Human Developmental Phases of Colorectal Cancer
  • Human Angiogenesis
  • Human Tumor Metastasis
  • Human Colorectal Cancer Metastasis
  • Human TGF β Pathway
  • Human ILK Signaling
  • Human Breast Cancer Regulations
  • Human Pancreatic Adenocarcinoma
  • Human Breast Cancer Regulations By Stathmin1,
  • Human BRCA1 PATHWAY

Pre-designed TaqMan® plates and products (384-well Micro fluidic Cards)

This product (TaqMan® Custom Array 384-well micro fluidic card) is able to perform up to 384 Taqman assays without robot, and analyze between 1 and 8 samples.

Some of the plates are:

  • Human Angiogenesis Array
  • Human Apoptosis Array
  • Human Endogenous Control Array
  • Human GPCR Array
  • Human Nuclear Receptor Array
  • Human Protein Kinase Array
  • Human and Mouse Alzheimer's Arrays
  • Human and Mouse Immune Array
  • Human and Rat Inflammation Array
  • Human and Rat Phosphodiesterase Array
  • TaqMan® Array MicroRNA Cards
  • TaqMan® Stem Cell Pluripotency Array

Additional information of these assays and other products are available at Applied Biosystems by life technologiesTM: Real-time PCR

Sequencing by capillary electrophoresis:

Automated DNA sequencing by Applied Biosystems uses the Sanger method. Once sequencing reaction is set up, the product is separated by capillary electrophoresis.

This technology enables to perform gene analysis with a high rate of mutation by the use of primers designed and optimized by the user as well as mitochondrial DNA study. Small (BigDye 1.1) 75 to 400pb fragments, and medium (BigDye 3.1) up to 1000pb fragments can be sequenced.

Complete gene amplicon sequence (RSA´s). VariantSEQr™ technology by M13 tailed primers.

Identification of microorganisms by 16S ribosomal sequencing.

Applications based on fragments analysis:

  • STR (short tandem repeats): genotyping method based on the study of microsatellite repeat.
  • Loss of Heterozygosity Analysis (LOH), which involves the use of microsatellites for detecting the loss of wild-type copy of a tumor suppressor gene in tumor samples.
  • Sequencing of 75-1050 base pairs from a DNA fragment, using one sequencing reaction. You can choose 2 different modes:
  Basic Service:
Only sequencing.
Full Service:
Every effort to get results.
Verification, quality, and concentration NO YES
Concentration adjustment NO YES
Sequencing reaction and electrophoresis YES YES
Visualization, edition, and assessment of the quality of results NO YES
Report of results by email YES YES
Free repetition of the sequence NO YES
Delivery time 3 working days 5 working days

Additional information of these assays and other products are available at Applied Biosystems by life technologiesTM: Real-time PCR

High-throughput genotyping:

Illumina BeadArray technology: it includes a wide range of types of arrays, and a large list of SNPs for inclusion on the arrays, including SNPs designed by the researcher.

For this type of studies, we recommend to contact the Project Manager of the Service. Your Project Manager will help ensure all parts involved on the design of your project and best suit your requirements. Send us a list of SNPs you want to analyze and we will confirm you the availability to be performed with an Illumina assay technology.

Some of the products from Illumina can be found below:

  • Arrays designed by the researcher :
    • Infinium iSelect HD Custom Genotyping BeadChip:
    • 24-sample format (3,000 to 68,000 assays)
    • 12-sample format (68,001 to 200,000 assays)
    • Semi-custom Human1M-Duo+ BeadChip (1.2 million assays)
  • Pre-designed Arrays:
    • Human1M-Duo DNA Analysis BeadChip Kits (1,2 million assays)
    • Over 10,000 SNPs and probes for the major histocompatibility complex (MHC) region.
    • Over 20,000 markers in more than 300 genes related to absorption, distribution, metabolism, and drugs excretion
    • About 60,000 positions with variability (CNV) and regions covering areas with undescribed CNVs. 60,800 SNPs can be added.
  • Other pre-designed arrays are: HumanCytoSNP-12 DNA Analysis BeadChip Kits, Focused Genotyping, Linkage Analysis, Whole-Genome Genotyping and Copy Number Analysis, Gene Regulation and Epigenetic Analysis, Array-Based Methylation Analysis, Gene Expression Analysis, Array-Based Transcriptome Analysis, FFPE Sample Analysis, Whole-Genome Gene Expression Analysis,…
 
Basic Service:
Only genotyping.
Full Service:
Higher exploitation of results.
Support on the experiment ‘s design
NO
YES
Verification, quality, and concentration
NO
YES
Concentration adjustment
NO
YES
Genomic amplification, enzymatic treatment, marking array
YES
YES
Report of results
YES
YES
Visualization, edition ,and assessment of the quality of results
NO
YES
Preliminary biostatistical analysis
NO
YES
Recording and backup of results
NO
YES

Next Generation Sequencing

SOLiD sequencing (This system will be available at very short notice).

All the services will be updated according to your requirements. Please contact us.

Our initial services include:

Complete exome resequencing and directed resequencing; whole exome resequencing, response of phenotype to drugs, medicines, or disease involved specific genes, detection of structural alterations and mutations.

  • Sample preparation for massive sequencing
  • Library preparation
  • DNA capture kits provided by Nimblegen, Agilent …
  • SOLID sequencing
  • Bioinformatic analysis in different formats:
    • Basic format: obtaining individual readings and associated quality assessment.
    • Full format: visualization and biological interpretation of results. Consensus sequences, list of SNPs, indels, inversions, CNVs. (according to the type of library).
  • Scientific and technical support to facilitate the interpretation of results